Restriction mapping is a method used to map an unknown segment of DNA by breaking it into pieces and then identifying the locations of the breakpoints. This method relies upon the use of proteins ...

Definition: A restriction enzyme is a DNA-cutting protein, found mainly in bacteria and archaea, that recognizes a specific short DNA sequence and cleaves the double helix at or near that site. By ...

Sau3AI is a type II restriction enzyme widely used for genetic manipulation, such as genome library construction. Sau3AI consists of two domains, the N-terminal domain (Sau3AI-N) and the C-terminal ...