High-throughput RNA sequencing (RNA-seq) is a widely used method for whole-transcriptome gene expression analysis, particularly in cancer cohort studies. The rapid development of diverse library ...

RNA library preparation can make or break whole transcriptome sequencing data acquisition. Speed, sensitivity, and scalability are key workflow considerations, but researchers often face bottlenecks ...

Saves labware, resources, and time Preserves frequently finite FFPE sample Increases the accuracy of tumor profiling by mitigating the bias caused by FFPE sample variability Compared to parallel or ...

New England Biolabs (NEB®) today announced the release of the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina®. This new kit incorporates an optimized template switching protocol— as ...

PCR cycle selection is a delicate balancing act. When preparing RNA-seq libraries, choosing too many cycles risks overcycling, which can compromise data quality and distort transcript representation.

Mariana Kiehl (Sr. Applications Scientist at Roche Sequencing & Life Science) describes the KAPA Total Prep FFPE workflow, a novel single-tube method for combined sequencing of DNA and RNA from FFPE ...

Although next-generation sequencing (NGS) keeps giving—exploring the human genome and revolutionizing our knowledge of health and disease—it needs to take a little, too. At the very least, NGS ...

BOULDER, Colo.--(BUSINESS WIRE)--Watchmaker Genomics, a supplier of innovative products for molecular analysis, announced the launch of the Watchmaker mRNA Library Prep Kit – designed to streamline ...

Common mitochondrial DNA (mtDNA) deletions are large structural variants in the mitochondrial genome that accumulate in metabolically active tissues with age and have been investigated in various ...

Next-generation sequencing (NGS) is increasingly employed to dive deeper into complex biological mechanisms in a myriad of disciplines. But as with most methodologies, the quality of the input ...