Fluorescence microscopy is a major workhorse in life sciences, biophysics, and physical chemistry, as it allows visualization with great specificity and sensitivity. In particular, the detection of ...

The resolution of optical microscopy is limited by diffraction limit and can only achieve a resolution of about 200 nm. In the past two decades, a series of technologies, such as STED, SIM, PALM/STORM ...

MINFLUX is a super-resolution microscopy protocol designed to improve the localizing of specific photon emitters in space. Developed in 2016 by a German team including super-resolution pioneer and ...

LMU researchers simplify the MINFLUX microscope and have succeeded in differentiating molecules that are extremely close together and tracking their dynamics. Only a few years ago, an ostensibly ...

Pushing the MINFLUX technique to higher spatial and temporal precision allows protein dynamics to be observed under physiological conditions. Scientists led by Nobel Laureate Stefan Hell at the Max ...

Distance markers: Polyprolines are relatively stiff polypeptides that can be used as intramolecular “rulers” to demonstrate MINFLUX optical resolutions. This image shows optically measured distances ...

Existing superresolution fluorescence microscopy methods such as STED and PALM have trouble imaging molecules with spatial resolution better than 20 to 40 nm. A new superresolution method called ...

(Nanowerk News) While many researchers use optical nanoscopes for their research, they are currently limited in their resolution due to how light diffracts. While techniques have been developed to ...